permeabilise

researchers permeabilise cells with triton x-100 to enable antibody penetration for immunofluorescence microscopy.

the protocol requires you to permeabilise the cell membrane before adding the primary antibody.

scientists permeabilise bacterial cells using lysozyme to extract their genetic material.

to perform intracellular staining, we must first permeabilise the fixed cells.

the detergent concentration determines how effectively you can permeabilise the plasma membrane.

over-permeabilisation can damage the cells, so the incubation time must be carefully controlled.

some protocols permeabilise tissues with saponin to preserve antigenicity.

the procedure will permeabilise the nuclear envelope, allowing transcription factors to enter.

researchers often permeabilise cells using digitonin for selective membrane permeabilisation.

the staining protocol includes a step to permeabilise cells with ethanol for intracellular dye loading.

it is essential to permeabilise the membrane without compromising cell viability.

we recommend permeabilising samples with methanol for flow cytometry analysis.

the antibody staining protocol involves permeabilising with tween-20 on fixed cell samples.